Coding
Part:BBa_K4467006:Design
Designed by: Eloise Paine Group: iGEM22_UNSW_Australia (2022-10-10)
SERK3B
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850
Illegal AgeI site found at 543 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 712
Design Notes
Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.
Source
This sequence for SERK3B in was Nicotiana benthamiana sourced from UniProt (E3VXE7).
References
UniProt (2022). Available at: https://www.uniprot.org/uniprotkb/E3VXE7/entry (Accessed: 12 October 2022).