Coding

Part:BBa_K4467006:Design

Designed by: Eloise Paine   Group: iGEM22_UNSW_Australia   (2022-10-10)


SERK3B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
    Illegal AgeI site found at 543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 712


Design Notes

Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.

Source

This sequence for SERK3B in was Nicotiana benthamiana sourced from UniProt (E3VXE7).

References

UniProt (2022). Available at: https://www.uniprot.org/uniprotkb/E3VXE7/entry (Accessed: 12 October 2022).